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《医学前沿(英文)》 2012年 第6卷 第4期 页码 366-375 doi: 10.1007/s11684-012-0214-6
Surveillance is critical for the prevention and control of infectious disease. China’s real-time web-based infectious disease reporting system is a distinguished achievement. However, many aspects of the current China Infectious Disease Surveillance System do not yet meet the demand for timely outbreak detection and identification of emerging infectious disease. PulseNet, the national molecular typing network for foodborne disease surveillance was first established by the Centers for Disease Control and Prevention of the United States in 1995 and has proven valuable in the early detection of outbreaks and tracing the pathogen source. Since 2001, the China CDC laboratory for bacterial pathogen analysis has been a member of the PulseNet International family; and has been adapting the idea and methodology of PulseNet to develop a model for a future national laboratory-based surveillance system for all bacterial infectious disease. We summarized the development progress for the PulseNet China system and discussed it as a model for the future of China’s national laboratory-based surveillance system.
关键词: infectious disease laboratory-based infectious disease surveillance pulse field gel electrophoresis multilocus sequencing typing PulseNet China
Application of proteomics in environmental science
Xiaona CHU, Jiangyong HU, Say Leong ONG,
《环境科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 393-403 doi: 10.1007/s11783-009-0145-x
关键词: proteomics environmental stress two dimensional (2D) gel electrophoresis mass spectrometry
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
《环境科学与工程前沿(英文)》 2012年 第6卷 第1期 页码 82-97 doi: 10.1007/s11783-011-0306-6
关键词: molecular biological technique microbial community denaturing gradient gel electrophoresis (DGGE) terminal restriction fragment length polymorphism (T-RFLP) environmental applications
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 40-47 doi: 10.1007/s11783-011-0304-8
关键词: sewage treatment plants polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) canonical correspondence analysis correspondence analysis
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
《环境科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 175-185 doi: 10.1007/s11783-011-0320-8
关键词: quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal component analysis (PCA) canonical correspondence analysis (CCA)
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
《工程(英文)》 doi: 10.1016/j.eng.2023.02.016
关键词: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel electrophoresis Mass spectrometry
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《医学前沿(英文)》 2012年 第6卷 第4期 页码 406-410 doi: 10.1007/s11684-012-0217-3
The esophagus is perfused directly by prominent arteries and may provide a more consistent tissue source for pulse oximetry. The goal of this study was to evaluate the sensitivity and accuracy of an esophageal pulse oximetry probe on patients during controlled hypoxemia in comparison to measurements obtained with conventional pulse oximetry (SpulseO2). Forty-five ASA I–II adult patients were included in this prospective observational study. Nellcor digital oximetric probes were placed on finger tips for SpulseO2 before anesthesia. After tracheal intubation, an esophageal probe was placed in the lower segment of the esophagus for esophageal oximetric monitoring (SoesO2). All patients were disconnected from the breathing circuit to establish a controlled hypoxemia, and were re-connected to the breathing circuit and ventilated with 100% oxygen immediately when SoesO2 dropped to 90%. Matched SoesO2 and SpulseO2 readings were recorded when SoesO2measurements were at 100%, 95%, 90% and the lowest reading. The time for SoesO2 and SpulseO2 to drop from 100% to 95%, 90% and return to 100% was recorded. Oxygen saturation from arterial blood samples (SartO2) was also measured at each time point respectively. The linear correlation coefficient of the regression analysis between SartO2 and SoesO2 was 0.954. The mean±2SD of the difference was 0.3%±4.3% for SoesO2vs. SartO2 and 6.8%±5.6% for SpulseO2vs. SartO2 (P<0.001). The 95% confidence interval for the absolute difference between SoesO2 and SartO2 was 0.3% to 0.7% and 6.2% to 7.4% between SpulseO2 and SartO2. The time to reach 90% saturation measured with SoesO2 was approximately 94 seconds earlier than the SpulseO2 (P<0.001). In conclusion, SoesO2 is more accurate and enables earlier detection of hypoxemia when compared to conventional pulse oximetry during hypoxemia for patients undergoing general anesthesia.
关键词: esophageal pulse oximetry conventional pulse oximetry hypoxemia
Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE
《环境科学与工程前沿(英文)》 2015年 第9卷 第2期 页码 324-333 doi: 10.1007/s11783-014-0627-3
关键词: aerobic granular sludge completely mixed activated sludge system (CMAS) continuous flow shear force filamentous bacteria polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)
Frequency-controlable sine signal based on PWM and its implementation on FPGA
Lianzhen HUANG, Jiangang LI, Dongjun ZHANG
《机械工程前沿(英文)》 2012年 第7卷 第3期 页码 322-328 doi: 10.1007/s11465-012-0312-9
A sine generation method that the different frequent sine signals can be generated by the different Pulse-Width Modulation (PWM) signals generated by Field-Programmable Gate Array (FPGA) through low-pass filter of fixed parameters was proposed. The method just takes a few FPGA resources and was proved feasible by the theory. The experiment results and theory analysis tally.
关键词: Pulse-Width Modulation (PWM) Field-Programmable Gate Array (FPGA) frequency controllable
可去除染料——N-聚糖多方法深入分析中的缺失环节 Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
《工程(英文)》 2023年 第26卷 第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu
《化学科学与工程前沿(英文)》 2017年 第11卷 第2期 页码 231-237 doi: 10.1007/s11705-017-1633-3
关键词: gelator Schiff base chiral recognition gel formation gel collapse
Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO
《结构与土木工程前沿(英文)》 2016年 第10卷 第2期 页码 189-197 doi: 10.1007/s11709-015-0326-6
关键词: cement composites photocatalytic TiO2 sol-gel C-S-H gel microstructure
Bin Xu, Toshiro Kaneko, Toshiaki Kato
《化学科学与工程前沿(英文)》 2019年 第13卷 第3期 页码 485-492 doi: 10.1007/s11705-019-1831-2
关键词: single-walled carbon nanotubes chirality-controlled synthesis pulse plasma chemical vapor deposition
A modified pulse charging method for lithium-ion batteries by considering stress evolution, charging
Yanfei ZHAO, Bo LU, Yicheng SONG, Junqian ZHANG
《结构与土木工程前沿(英文)》 2019年 第13卷 第2期 页码 294-302 doi: 10.1007/s11709-018-0460-z
关键词: fast charging method pulse charging stress evolution charging time capacity utilization
LIU Bin, SU Wanhua, WANG Hui, HUANG Haozhong
《能源前沿(英文)》 2007年 第1卷 第4期 页码 420-427 doi: 10.1007/s11708-007-0061-7
关键词: combustion compression homogenous in-cylinder modulated multi-pulse
标题 作者 时间 类型 操作
PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China
null
期刊论文
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
期刊论文
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
期刊论文
Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
期刊论文
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
期刊论文
Esophageal pulse oximetry is more accurate and detects hypoxemia earlier than conventional pulse oximetry
null
期刊论文
Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system
Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE
期刊论文
Frequency-controlable sine signal based on PWM and its implementation on FPGA
Lianzhen HUANG, Jiangang LI, Dongjun ZHANG
期刊论文
Visual chiral recognition of 1,1′-binaphthol through enantioselective collapse of gel based on an amphiphilic
Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu
期刊论文
Microstructural and photocatalytic characterization of cement-paste sol-gel synthesized titanium dioxide
Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO
期刊论文
Improvement in growth yield of single-walled carbon nanotubes with narrow chirality distribution by pulse
Bin Xu, Toshiro Kaneko, Toshiaki Kato
期刊论文
A modified pulse charging method for lithium-ion batteries by considering stress evolution, charging
Yanfei ZHAO, Bo LU, Yicheng SONG, Junqian ZHANG
期刊论文